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pCH001
(Plasmid #47953)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47953 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS306
  • Backbone manufacturer
    ATCC
  • Total vector size (bp) 4373
  • Vector type
    Yeast Expression ; Tet inducible expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Promoter T3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F20
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tetO2 inducible expression of GFP.

The selection marker genes have some differences to the published sequence but this does not affect the functioning of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCH001 was a gift from Attila Becskei (Addgene plasmid # 47953 ; http://n2t.net/addgene:47953 ; RRID:Addgene_47953)
  • For your References section:

    Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution. Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, Becskei A. Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687. 10.1038/ncomms1687 PubMed 22353713