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(Plasmid #48250)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 48250 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
  • Promoter CMV

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

pHyper was cloned by replacing EGFP with the Hyper sequence in the pEGFP-C1 vector (Clontech). To make pSypHer, the C199S mutation was made with the QuikChange II site-directed mutagenesis kit using the following oligos:
5′-agatggtcactctttgcgcgat-3′ (forward)
5′-atcgcgcaaagagtgaccatct-3′ (reverse)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SypHer was a gift from Nicolas Demaurex (Addgene plasmid # 48250 ; ; RRID:Addgene_48250)
  • For your References section:

    Dynamic regulation of the mitochondrial proton gradient during cytosolic calcium elevations. Poburko D, Santo-Domingo J, Demaurex N. J Biol Chem. 2011 Apr 1;286(13):11672-84. doi: 10.1074/jbc.M110.159962. Epub 2011 Jan 11. 10.1074/jbc.M110.159962 PubMed 21224385