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SypHer mt
(Plasmid #48251)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48251 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 48251-D50 50 μg of DNA in Tris buffer $495
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DNA 48251-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHyPer-dMito
  • Backbone manufacturer
    Evrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SypHer
  • Species
    Synthetic
  • Insert Size (bp)
    1400
  • Mutation
    C199S
  • Promoter CMV
  • Tag / Fusion Protein
    • 2 mitochondrial targeting sequences (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The C199S mutation was made in pHyper-mito with the QuikChange II site-directed mutagenesis kit using the following oligos:
5′-agatggtcactctttgcgcgat-3′ (forward)
5′-atcgcgcaaagagtgaccatct-3′ (reverse)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SypHer mt was a gift from Nicolas Demaurex (Addgene plasmid # 48251 ; http://n2t.net/addgene:48251 ; RRID:Addgene_48251)

  • For your References section:

    Dynamic regulation of the mitochondrial proton gradient during cytosolic calcium elevations. Poburko D, Santo-Domingo J, Demaurex N. J Biol Chem. 2011 Apr 1;286(13):11672-84. doi: 10.1074/jbc.M110.159962. Epub 2011 Jan 11. 10.1074/jbc.M110.159962 PubMed 21224385
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