PurposeCre-dependant (flex) rabies glycoprotein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48333||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7663
Modifications to backbonelox2272(c)-loxP(c)-SalI-AscI-lox2272-loxP cassette was inserted between CAG promoter and WPRE-hGHpA.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Gene/Insert nameRabies Glycoprotein
Insert Size (bp)1575
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (not destroyed)
- 3′ cloning site Asc1 (not destroyed)
- 5′ sequencing primer TriEXUP.5
- 3′ sequencing primer pWPTrev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CAG-Flex-RG was a gift from Liqun Luo (Addgene plasmid # 48333)
For your References section:Dissecting local circuits: parvalbumin interneurons underlie broad feedback control of olfactory bulb output. Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A. Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027. Epub 2013 Nov 14. 10.1016/j.neuron.2013.08.027 PubMed 24239125