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roGFP_iL_pQE30
(Plasmid #48633)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48633 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQE30
  • Backbone size w/o insert (bp) 3418
  • Total vector size (bp) 4174

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    M15
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    roGFP1
  • Species
    jellyfish
  • Insert Size (bp)
    756
  • Mutation
    insertion L147B
  • Tag / Fusion Protein
    • HisTag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pQEForward
  • 3′ sequencing primer pQEReverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    James Remington U. Oregon USA
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmids needs to be amplified and maintained in M15 at all times as this strain has the pREP4 repressor vector that prevents undesired expression (it's important to maintain the plasmid when the protein is not expressed even if it's believed to be inert for the bacteria, to avoid selective pressure in favour of recombinant versions of the gene).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    roGFP_iL_pQE30 was a gift from David Ron (Addgene plasmid # 48633 ; http://n2t.net/addgene:48633 ; RRID:Addgene_48633)
  • For your References section:

    Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox. Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, Melo EP, Kaminski CF, Ron D. J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155. 10.1083/jcb.201211155 PubMed 23589496