ERrefGFP1_pCDNA3
(Plasmid #48635)

• Purpose: Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
• Depositing Lab: David Ron
• Publication: Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.

Backbone

• Vector backbone: pCDNA3 derivative
• Backbone size w/o insert (bp): 5393
• Total vector size (bp): 6191
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERrefGFP1
• Species: Jellyfish
• Insert Size (bp): 798
• Mutation: C147S,C204Q
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMVforward
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: James Remington U. Oregon USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry