Purpose(Empty Backbone) Expression of TALEN proteins with FokI-RRR domain
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48636||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5798
Modifications to backboneCloned in TALEN expression sequence
- Promoter CMV
/ Fusion Proteins
- HA-1 (N terminal on insert)
- HA-2 (N terminal on insert)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer Tal Seq FokI Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2TAL3-RRR was a gift from David Grunwald (Addgene plasmid # 48636 ; http://n2t.net/addgene:48636 ; RRID:Addgene_48636)
For your References section:Targeting human microRNA genes using engineered Tal-effector nucleases (TALENs). Hu R, Wallace J, Dahlem TJ, Grunwald DJ, O'Connell RM. PLoS One. 2013 May 7;8(5):e63074. doi: 10.1371/journal.pone.0063074. Print 2013. 10.1371/journal.pone.0063074 PubMed 23667577