SNF-5 pXOOM
(Plasmid
#48697)
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PurposeExpressed SNF-5 in Xenopus oocytes or mammalian cells
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Depositing Lab
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Depositing OrganizationRosalind Franklin University of Medicine and Science
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 48697-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 48697-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepXOOM
- Backbone size w/o insert (bp) 5052
- Total vector size (bp) 7239
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Vector typeMammalian Expression ; Xenopus Oocyte Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesnf-5
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Alt nameCeNAT5
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SpeciesC. elegans (nematode)
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MutationK42E (when compared to NP_496735.1)
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Entrez Genesnf-5 (a.k.a. CELE_Y46G5A.30)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer ATG GCC GAT TCG GGC AGC AAT GA
- 3′ sequencing primer T TAA TAA ACT TGA TGA TAT GTA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 48697-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 48697-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SNF-5 pXOOM was a gift from Dmitri Boudko (Addgene plasmid # 48697 ; http://n2t.net/addgene:48697 ; RRID:Addgene_48697) -
For your References section:
An SLC6 transporter of the novel B(0,)- system aids in absorption and detection of nutrient amino acids in Caenorhabditis elegans. Metzler R, Meleshkevitch EA, Fox J, Kim H, Boudko DY. J Exp Biol. 2013 Aug 1;216(Pt 15):2843-57. doi: 10.1242/jeb.081497. Epub 2013 Apr 11. 10.1242/jeb.081497 PubMed 23580723
