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pTAL7b
(Plasmid #48706)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48706 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZErO-2
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 7210
  • Vector type
    Mammalian Expression, TALEN
  • Promoter CAG
  • Selectable markers
    Blasticidin
  • Tag / Fusion Protein
    • FokI nuclease (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The truncated TALE backbone and FokI wild-type sequences were PCR-amplified from plasmid pCAG-TAL-linker-IX_Fokwt kindly provided by Dr. Ralf Kühn.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

Article describing generation and use of this plasmid is currently in revision

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAL7b was a gift from Boris Greber (Addgene plasmid # 48706)
  • For your References section:

    A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models. Frank S, Skryabin BV, Greber B. BMC Genomics. 2013 Nov 9;14(1):773. 10.1186/1471-2164-14-773 PubMed 24206569