Purpose(Empty Backbone) TALEN expression in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48706||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7210
Vector typeMammalian Expression, TALEN
- Promoter CAG
/ Fusion Protein
- FokI nuclease (C terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- 5′ sequencing primer CGCTTGGAGGAATGCTCTGA
- 3′ sequencing primer AAGCGAGAGCGACCAGATGAT (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe truncated TALE backbone and FokI wild-type sequences were PCR-amplified from plasmid pCAG-TAL-linker-IX_Fokwt kindly provided by Dr. Ralf Kühn.
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Article describing generation and use of this plasmid is currently in revision
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTAL7b was a gift from Boris Greber (Addgene plasmid # 48706 ; http://n2t.net/addgene:48706 ; RRID:Addgene_48706)
For your References section:A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models. Frank S, Skryabin BV, Greber B. BMC Genomics. 2013 Nov 9;14(1):773. 10.1186/1471-2164-14-773 PubMed 24206569