pPy-CAG-GFP-IRES-Pac
(Plasmid
#48759)
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PurposeCAG promoter driving GFP-IRES-Puromycin Acetyl Transferase
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Depositing Lab
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Depositing OrganizationUniversity of Cambridge
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48759 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 48759-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 48759-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneBluescript
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 7300
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Vector typeMammalian Expression ; pPy
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)700
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CAG forward
- 3′ sequencing primer pA backward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 48759-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 48759-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPy-CAG-GFP-IRES-Pac was a gift from Austin Smith (Addgene plasmid # 48759 ; http://n2t.net/addgene:48759 ; RRID:Addgene_48759) -
For your References section:
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A. Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012. 10.1016/j.cell.2013.03.012 PubMed 23582324
Map uploaded by the depositor.
