pCAG:GPI-GFP
(Plasmid #32601)

• Depositing Lab: Anna-Katerina Hadjantonakis
• Publication: Rhee et al Genesis. 2006 Apr;44(4):202-18.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GPI-GFP
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: 5′ GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GTG AGC AAG GGC GAG GAG CTG)
• 3′ sequencing primer: 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG TCC

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry