Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCAG:myr-Venus
(Plasmid #32602)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32602 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myr-Venus
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GTG AGC AAG GGC GAG GAG CTG
  • 3′ sequencing primer GTC ATG AAT TCT TAC TTG TAC AGC TCG TCC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate myristoylated fluorescent fusion proteins, an N-terminal myristoylation tag
derived from Src was generated by adding the sequences MGSSKSKPK to the N-terminus of
any given fluorescent protein. This was achieved by using the following oligonucleotide: 5′
GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA
AAG GTG AGC AAG GGC GAG GAG CTG). The GFP and Venus coding sequences were
amplified from pEGFP-N1 (BD Biosciences, San Jose, CA) and pCS2-Venus (Nagai et al.,
2002) to generate myr-GFP and myr-Venus, respectively, by high-fidelity polymerase chain
reaction (PCR) using Pfx Polymerase (Invitrogen, La Jolla, CA) with the 5′ myristoylation
primer combined with a 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG
TCC) primer, respectively. The resulting product was cloned into the EcoRI site of pCAGGS
to generate pCX::myr-EGFP and pCX::myr-Venus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG:myr-Venus was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32602 ; http://n2t.net/addgene:32602 ; RRID:Addgene_32602)
  • For your References section:

    In vivo imaging and differential localization of lipid-modified GFP-variant fusions in embryonic stem cells and mice. Rhee JM, Pirity MK, Lackan CS, Long JZ, Kondoh G, Takeda J, Hadjantonakis AK. Genesis. 2006 Apr;44(4):202-18. 10.1002/dvg.20203 PubMed 16604528