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pCAG:myr-mRFP1
(Plasmid #32604)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32604 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4698
  • Modifications to backbone
    pCX::myr-RFP was generated by PCR of mRFP1 from pRSET-mRFP1. The resulting PCR product was cloned into a pCRII-TOPO (Invitrogen) and the insert excised with EcoRI and cloned into pCAGGS (Niwa et al., 1991).
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myr-mRFP1
  • Species
    jellyfish
  • Promoter pCAGGs

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GCC TCC TCC GAG GAC GTC ATC AAG G
  • 3′ sequencing primer CAA GC TTC GAA TTC TTA GGC GCC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12) https://www.ncbi.nlm.nih.gov/pubmed/12060735 .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG:myr-mRFP1 was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32604 ; http://n2t.net/addgene:32604 ; RRID:Addgene_32604)
  • For your References section:

    Dual transgene strategy for live visualization of chromatin and plasma membrane dynamics in murine embryonic stem cells and embryonic tissues. Nowotschin S, Eakin GS, Hadjantonakis AK. Genesis. 2009 May;47(5):330-6. 10.1002/dvg.20500 PubMed 19358158