pRGM10
(Plasmid #48790)

• Purpose: I-SceI DSB repair reporter cassette
• Depositing Lab: Michael Glickman
• Publication: Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. doi: 10.1111/j.1365-2958.2010.07463.x. Epub 2010 Nov 24.

Backbone

• Vector backbone: pRGM9
• Vector type: DSB repair reporter

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use Kanamycin at 40ug/ml and grow cultures for a longer time than normal (approximately 20 hours)
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: lacZ(I-SceI)
• Insert Size (bp): 2570

Gene/Insert 2

• Gene/Insert name: lacZ-deltaN
• Insert Size (bp): 2439

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry