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pICXC3H
(Plasmid #49022)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49022 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPICZ
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5050
  • Modifications to backbone
    modified for compatibility with FX cloning and added tags/fusion proteins
  • Vector type
    Yeast Expression
  • Promoter AOX1
  • Selectable markers
    Zeocin
  • Tags / Fusion Proteins
    • 3C protease site (PreScission site) (C terminal on backbone)
    • decaHis (10x His) (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Bleocin (Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AOX1-fwd (5'-GACTGGTTCCAATTGACAAGC-3')
  • 3′ sequencing primer AOX1-rev (5'-GCAAATGGCATTCTGACATCC-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICXC3H was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49022 ; http://n2t.net/addgene:49022 ; RRID:Addgene_49022)
  • For your References section:

    A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291