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A versatile and efficient high-throughput cloning tool for structural biology.
Geertsma ER, Dutzler R
Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46858pINIT_catFX sequencing vector for subcloning, chloramphenicol marker
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46973pINIT_kanFX sequencing vector for subcloning, kanamycin marker
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46974pINIT_tetFX sequencing vector for subcloning, tetracycline marker
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47064p7XNH3FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site
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47065p7XC3HFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag
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47066p7XC3GHFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
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47067pBXNH3FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site
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47068pBXC3HFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag
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47069pBXNH3CAFX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag
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47070pBXC3GHFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
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47071pBXCA3GHFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags
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47072pBXCA3HFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag
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47073pBXNAC3GHFX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags
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47074pBXNAC3HFX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag
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47075pBXNH3AFX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag
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47077pREXC3GHFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
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47078pREXC3HFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tags
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47079pREXNH3FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site
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49020pICXNH3FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site
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49021pICXNHG3FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site
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49022pICXC3HFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag
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49023pICXC3GHFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag
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49024pYEXNH3FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site
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49025pYEXNHG3FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site
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49026pYEXC3HFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag
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49027pYEXC3GHFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag
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49028pcDXNSM3FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site
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49029pcDXNSMG3FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site
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49030pcDXC3MSFX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide
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49031pcDXC3GMSFX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide
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49032pTLNXFX cloning Xenopus oocyte expression vector with SP6 promoter
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49033pTLNXA7FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence
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