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pcDXNSM3
(Plasmid #49028)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49028 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5855
  • Modifications to backbone
    modified for compatibility with FX cloning and added tags/fusion proteins
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tags / Fusion Proteins
    • streptavidin binding peptide (SBP) (N terminal on backbone)
    • MYC (N terminal on backbone)
    • 3C protease site (PreScission site) (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDXNSM3 was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49028 ; http://n2t.net/addgene:49028 ; RRID:Addgene_49028)
  • For your References section:

    A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291