Purpose(Empty Backbone) FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49033||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerLorenz et al, 1996 (PubMed ID: 8917596)
- Backbone size (bp) 5306
Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
Vector typeXenopus oocytes expression
- Promoter SP6
/ Fusion Protein
- chicken alpha7 signal sequence (N terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Copy numberLow Copy
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer M13pUC-rev (Common Sequencing Primers)
Terms and Licenses
Constructed by Dr. Iwan Zimmermann, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTLNXA7 was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49033 ; http://n2t.net/addgene:49033 ; RRID:Addgene_49033)
For your References section:A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291