Purpose(Empty Backbone) FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49029||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6578
Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
Vector typeMammalian Expression
- Promoter CMV
/ Fusion Proteins
- streptavidin binding peptide (SBP) (N terminal on backbone)
- MYC (N terminal on backbone)
- EGFP (N terminal on backbone)
- 3C protease site (PreScission site) (N terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDXNSMG3 was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49029 ; http://n2t.net/addgene:49029 ; RRID:Addgene_49029)
For your References section:A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291