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pBXNAC3GH
(Plasmid #47073)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47073 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD24
  • Backbone manufacturer
    LM Guzman
  • Backbone size (bp) 5779
  • Modifications to backbone
    modified for compatibility with FX cloning and added tags/fusion proteins
  • Vector type
    Bacterial Expression
  • Promoter araBAD
  • Tags / Fusion Proteins
    • Avi-tag (N terminal on backbone)
    • 3C protease site (PreScission site) (C terminal on backbone)
    • GFP (C terminal on backbone)
    • decaHIS (10x His) (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBXNAC3GH was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 47073 ; http://n2t.net/addgene:47073 ; RRID:Addgene_47073)
  • For your References section:

    A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291