Purpose(Empty Backbone) FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47077||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerEric R Geertsma & Bert Poolman
- Backbone size (bp) 6247
Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
Vector typeLactococcus lactis expression
- Promoter NIS (nisin)
/ Fusion Proteins
- 3C protease site (C terminal on backbone)
- GFP (C terminal on backbone)
- His (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pREXC3GH was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 47077 ; http://n2t.net/addgene:47077 ; RRID:Addgene_47077)
For your References section:A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291