Flag‐mTim3 (del.Cyto)
(Plasmid #49209)

• Purpose: expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)
• Depositing Lab: Lawrence Kane
• Publication: Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. Epub 2011 Aug 1.

Backbone

• Vector backbone: pCDEF3-CD8SS-Flag
• Total vector size (bp): 6872
• Modifications to backbone: a pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tim3 (del.Cyto)
• Alt name: hepatitis A virus cellular receptor 2
• Alt name: HAvcr2
• Alt name: Tim-3
• Species: M. musculus (mouse)
• Mutation: cytosolic domain deleted, no Tim3 signal sequence; Y218F compared to NP_599010.4
• Entrez Gene: Havcr2 (a.k.a. TIM-3, Tim3, Timd3)
• Promoter: Ef1a
• Tags / Fusion Proteins:
  • CD8 signal sequence (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry