Flag‐mTim3
(Plasmid #49208)

• Purpose: expresses Flag tagged mouse Tim3
• Depositing Lab: Lawrence Kane
• Publication: Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. Epub 2011 Aug 1.

Backbone

• Vector backbone: pCDEF3-CD8SS-Flag
• Backbone manufacturer: Kane Lab
• Backbone size w/o insert (bp): 6094
• Total vector size (bp): 7045
• Modifications to backbone: a pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tim3
• Alt name: hepatitis A virus cellular receptor 2
• Alt name: HAvcr2
• Alt name: Tim-3
• Species: M. musculus (mouse)
• Mutation: no Tim3 signal sequence
• Entrez Gene: Havcr2 (a.k.a. TIM-3, Tim3, Timd3)
• Promoter: EF1a
• Tags / Fusion Proteins:
  • signal sequence (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length Tim-3 (without the signal sequence) was then amplified with specific primers and cloned into a mammalian expression vector containing a human CD8α signal sequence and Flag epitope tag.