pJM126
(Plasmid #49339)

• Purpose: bacterial expression of RFA1, RFA2, and RFA3
• Depositing Lab: Steven Brill
• Publication: He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

Backbone

• Vector backbone: pET11a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 9300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: RFA3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 730
• Entrez Gene: RFA3 (a.k.a. YJL173C, RPA14, RPA3)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: pBRrevBam

Gene/Insert 2

• Gene/Insert name: RFA2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1100
• Entrez Gene: RFA2 (a.k.a. YNL312W, BUF1, RPA2, RPA32)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: NA

Gene/Insert 3

• Gene/Insert name: RFA1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1900
• Entrez Gene: RFA1 (a.k.a. YAR007C, BUF2, FUN3, RPA1, RPA70)
• Promoter: T7

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: NA
• 3′ sequencing primer: T7 terminal

Resource Information

• Supplemental Documents:
  • Purification of RPA
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry