pTRCHisB-TRF2_M
(Plasmid #50490)

• Purpose: E. Coli N-term HexHis Tagged Protein Expression Vector
• Depositing Lab: Jack Griffith
• Publication: Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. Epub 2014 Aug 21.

Backbone

• Vector backbone: pTrcHisB
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 4378
• Total vector size (bp): 4591
• Modifications to backbone: See putative plasmid sequence
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRF2 Myb
• Alt name: TERF2
• Alt name: TRBF2
• Alt name: telomeric repeat binding factor 2
• Species: H. sapiens (human)
• Mutation: Contains amino acids 438-500
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)
• Promoter: pTrc
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • T7 tag (N terminal on backbone)
  • TEV protease site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: AAT CTG TGT GGG CAC TCG
• 3′ sequencing primer: CTT CTG CGT TCT GAT TTA ATC TG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This vector is modified from those in use by Dr. Gilson and his colleagues.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This set of vectors has been engineered to reintroduce Alanine 434, which is omitted in most TRF2 vectors, and to generate TRF2 and TRF2 mutants with identical N-terminal tags.