pGL1.7XX
(Plasmid
#49344)
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Purposeluciferase reporter with mouse Bmp2 promoter
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Depositing Lab
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Depositing OrganizationUniversity of South Florida
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 49344-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 49344-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL2 basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5598
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBmp2 distal promoter
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Alt nameBmp2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1702
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Mutationnt –1237 to +471
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GenBank ID12156
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Entrez GeneBmp2 (a.k.a. Bmp2a)
- Promoter Bmp2 distal promoter
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer LucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 49344-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 49344-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL1.7XX was a gift from Melissa Rogers (Addgene plasmid # 49344 ; http://n2t.net/addgene:49344 ; RRID:Addgene_49344) -
For your References section:
An evolutionary and molecular analysis of Bmp2 expression. Abrams KL, Xu J, Nativelle-Serpentini C, Dabirshahsahebi S, Rogers MB. J Biol Chem. 2004 Apr 16;279(16):15916-28. Epub 2004 Feb 2. 10.1074/jbc.M313531200 PubMed 14757762
Map uploaded by the depositor.
