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pGLB2 5'3'
(Plasmid #49346)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 49346 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Bmp2 distal promoter
  • Alt name
    Bmp2
  • Alt name
    Construct B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Entrez Gene
    Bmp2 (a.k.a. AI467020, Bmp2a)
  • Promoter -
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Bmp2 3' UTR
  • Alt name
    Bmp2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2215
  • Entrez Gene
    Bmp2 (a.k.a. AI467020, Bmp2a)
  • Promoter -

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site StyI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer LucF
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

5' region contains nt –1,237 to 471 relative to distal transcription start.
3' region contains nt 9574-11604 relative to distal transcription start and contains two Bmp2 polyadenylation signals yielding 3’UTRs of 870 and 1185 nt (Liu et al. PMID 18703506) and downstream flanking sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGLB2 5'3' was a gift from Melissa Rogers (Addgene plasmid # 49346 ; http://n2t.net/addgene:49346 ; RRID:Addgene_49346)
  • For your References section:

    Conservation of Bmp2 post-transcriptional regulatory mechanisms. Fritz DT, Liu D, Xu J, Jiang S, Rogers MB. J Biol Chem. 2004 Nov 19;279(47):48950-8. Epub 2004 Sep 9. 10.1074/jbc.M409620200 PubMed 15358784