|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49388||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression, Cre/Lox ; acceptor vector
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Copy numberHigh Copy
Alt namev-akt murine thymoma viral oncogene homolog 1
SpeciesH. sapiens (human)
Insert Size (bp)1487
Mutationtga to cca at nt 1725 with respect to GenBank ID BC000479
Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
hAkt1 was transferred from the donor vector (pDNR-hAkt1, Addgene plasmid #49387) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
When the recombinant construct is expressed in mammalian cells, EGFP is expressed from the immediate early promoter of cytomegalovirus (pCMV IE) as a 3' fusion to Akt1, through intron splicing.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLPS-hAkt1-gfp was a gift from Randall McKinnon (Addgene plasmid # 49388 ; http://n2t.net/addgene:49388 ; RRID:Addgene_49388)
Map uploaded by the depositor.