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pBlue-TAL
(Plasmid #49401)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49401 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS+
  • Modifications to backbone
    Synthetic 2030 bp DNA fragment encoding truncated TAL-effector N- and C-terminus and FokI nuclease domain was subcloned into a pBluescript KS+ plasmid digested with Pvu II.
  • Vector type
    Insect Expression, TALEN
  • Selectable markers
    lacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • Terms and Licenses

Depositor Comments

Additional reference: The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly. Takasu Y. et al. Methods 2014. PMID: 24565747

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBlue-TAL was a gift from Michal Zurovec (Addgene plasmid # 49401)
  • For your References section:

    Efficient TALEN Construction for Bombyx mori Gene Targeting. Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu H, Tamura T, Zurovec M. PLoS One. 2013 Sep 18;8(9):e73458. doi: 10.1371/journal.pone.0073458. 10.1371/journal.pone.0073458 PubMed 24058473