HA-HIF1a (S668A)
(Plasmid #49463)

• Purpose: HA-tagged HIF-1a Ser668 resuide mutated to Ala
• Depositing Lab: Wafik El-Deiry
• Publication: Warfel et al Cell Cycle. 2013 Oct 25;12(23).

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5433
• Total vector size (bp): 7938
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hypoxia inducible factor 1 alpha
• Alt name: HIF-1a
• Species: H. sapiens (human)
• Insert Size (bp): 2505
• Mutation: Serine 668 to Alanine
• GenBank ID: NM_001530
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Promoter: T7
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (unknown if destroyed)
• 3′ cloning site: ECOR1 (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid 18949: HA-HIF1alpha-pcDNA3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry