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pETM6
(Plasmid #49795)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49795 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-Duet
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 5000
  • Modifications to backbone
    AvrII was inserted upstream of the T7 promoter, XbaI was inserted between the operator (lacO) and ribosome binding site (RBS), SpeI was between the multiple-cloning site (MCS) and T7 terminator, and NheI was placed downstream of the T7 terminator
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • S-tag (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6 was a gift from Mattheos Koffas (Addgene plasmid # 49795 ; http://n2t.net/addgene:49795 ; RRID:Addgene_49795)
  • For your References section:

    ePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli. Xu P, Vansiri A, Bhan N, Koffas MA. ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. Epub 2012 May 4. 10.1021/sb300016b PubMed 23651248