PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerJoung lab (Addgene plasmid #49237)
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert name3x FLAG Tet1CD RH-4
Alt nameRHOXF2 site #4
SpeciesH. sapiens (human)
Entrez GeneRHOXF2 (a.k.a. CT107, PEPP-2, PEPP2, THG1)
- Promoter EF1a
/ Fusion Protein
- 3x FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (unknown if destroyed)
- 3′ cloning site BsmBI (unknown if destroyed)
- 5′ sequencing primer EF1a-F 5'-TCAAGCCTCAGACAGTGGTTC-3'
- 3′ sequencing primer OJA199 5'-CACCATCACAGCAGTTGG-3' (Common Sequencing Primers)
TALE target site is: TCAGCACAGCAGCTTCCCCC.
See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MLM3713 was a gift from Keith Joung (Addgene plasmid # 49946 ; http://n2t.net/addgene:49946 ; RRID:Addgene_49946)
For your References section:Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK. Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726. 10.1038/nbt.2726 PubMed 24108092