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pET28a-PACT
(Plasmid #50352)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50352 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 6349
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PACT
  • Alt name
    PRKRA
  • Alt name
    protein kinase, interferon-inducible double stranded RNA dependent activator
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRKRA (a.k.a. DYT16, HSD14, PACT, RAX)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The PACT CDS was amplified from a cDNA mixture of total RNA extracted from human HeLa cells by PCR using primers with restriction enzyme sites of BamHI and SalI. The amplified fragment was digested with same enzyme sites and cloned into pET-28a vector amplified using primers with same enzyme sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-PACT was a gift from Kumiko Ui-Tei (Addgene plasmid # 50352 ; http://n2t.net/addgene:50352 ; RRID:Addgene_50352)
  • For your References section:

    Distinguishable in vitro binding mode of monomeric TRBP and dimeric PACT with siRNA. Takahashi T, Miyakawa T, Zenno S, Nishi K, Tanokura M, Ui-Tei K. PLoS One. 2013 May 2;8(5):e63434. doi: 10.1371/journal.pone.0063434. Print 2013. 10.1371/journal.pone.0063434 PubMed 23658827