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PF10_0166-bio-His
(Plasmid #50801)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50801 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher
  • Backbone size w/o insert (bp) 6670
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PF10_0166
  • Species
    H. sapiens (human); P. falciparum
  • Insert Size (bp)
    875
  • Mutation
    all potential N-linked glycosylation sites (N-X-S/T, where X is not proline) were systematically removed by substituting alanine for serine/threonine at these sites.
  • Promoter CMV
  • Tag / Fusion Protein
    • ratCD4d3+4,biotinylation sequence, 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBVori-F (5'-CCACTCAGTGTTGGCAAATG-3')
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada
  • Terms and Licenses

Depositor Comments

Made by gene synthesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PF10_0166-bio-His was a gift from Gavin Wright (Addgene plasmid # 50801)
  • For your References section:

    Towards a comprehensive Plasmodium falciparum merozoite cell surface and secreted recombinant protein library. Zenonos ZA, Rayner JC, Wright GJ. Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93. 10.1186/1475-2875-13-93 PubMed 24620899