PurposeExpresses enzymatically monobiotinylated full length PFD1130w
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50823||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher
- Backbone size w/o insert (bp) 6670
Modifications to backboneAddition of CD4, biotinylation sequence, 6xhis tags
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1019
Mutationall potential N-linked glycosylation sites (N-X-S/T, where X is not proline) were systematically removed by substituting alanine for serine/threonine at these sites.
- Promoter CMV
/ Fusion Protein
- ratCD4d3+4,biotinylation sequence, 6xHis (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBVori-F (5'-CCACTCAGTGTTGGCAAATG-3') (Common Sequencing Primers)
Made by gene synthesis
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PFD1130w-bio-His was a gift from Gavin Wright (Addgene plasmid # 50823 ; http://n2t.net/addgene:50823 ; RRID:Addgene_50823)
For your References section:Towards a comprehensive Plasmodium falciparum merozoite cell surface and secreted recombinant protein library. Zenonos ZA, Rayner JC, Wright GJ. Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93. 10.1186/1475-2875-13-93 PubMed 24620899