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p14-9VNA5
(Plasmid #50931)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50931 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBiFC-VN173
  • Backbone manufacturer
    Chang-Deng Hu (Addgene Plasmid# 22010)
  • Backbone size w/o insert (bp) 5216
  • Total vector size (bp) 5879
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SRP14 and SRP9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    677
  • Mutation
    Amino acids 96-100 of SRP14 changed to alanine
  • Entrez Gene
    SRP14 (a.k.a. ALURBP)
  • Entrez Gene
    SRP9 (a.k.a. ALURBP)
  • Promoter CMV
  • Tags / Fusion Proteins
    • flag tag (N terminal on backbone)
    • VN tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p14-9VNA5 was a gift from Katharina Strub (Addgene plasmid # 50931 ; http://n2t.net/addgene:50931 ; RRID:Addgene_50931)