pGEX RalB G23V ΔC
(Plasmid
#50995)
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PurposeExpresses RalB G23V ΔC in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50995 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-4T2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRALB
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SpeciesH. sapiens (human)
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MutationG23V ΔC
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Entrez GeneRALB
- Promoter tac_promoter
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX_5_primer
- 3′ sequencing primer pGEX_3_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOrfeome
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX RalB G23V ΔC was a gift from Anna Sablina (Addgene plasmid # 50995 ; http://n2t.net/addgene:50995 ; RRID:Addgene_50995) -
For your References section:
The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response. Simicek M, Lievens S, Laga M, Guzenko D, Aushev VN, Kalev P, Baietti MF, Strelkov SV, Gevaert K, Tavernier J, Sablina AA. Nat Cell Biol. 2013 Oct;15(10):1220-30. doi: 10.1038/ncb2847. Epub 2013 Sep 22. 10.1038/ncb2847 PubMed 24056301
