pSIR-GFP
(Plasmid #51134)

• Purpose: (Empty Backbone) Self-inactivating retrovirus vector containing the GFP gene
• Depositing Lab: Hodaka Fujii
• Publication: Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014.

Backbone

• Vector backbone: pSIR
• Backbone manufacturer: Clontech
• Backbone size (bp): 8089
• Modifications to backbone: Replacement of the neomycin resistance gene with GFP
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: We have recognized that rare recombination evens may occur and give rise to clones with deletions (ca. 5 kb in size). If you encounter this problem, try picking more colonies (at least 10). Use 1 ml for miniprep and double-digest with Xho I and Nco I. Correct clones have 7.0 kbp + 890 bp (wrong ones are around 5 kbp). Please use the remaining 4 ml culture of the correct clones for expansion into a large scale. You may find dark colonies and pale colonies on the plate, and the pale ones are correct clones in our hand. In cloning of gBlock, the plasmid will be digested with Xho I and Hind III, for example, and the 8 kbp fragment will be purified. Therefore, the deleted clones will be removed during the purification step even if they are contaminated in the sample.
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry