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DsRed-p150 217–548
(Plasmid #51146)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51146 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDsRed-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCTN1
  • Alt name
    p150Glued
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    997
  • Mutation
    Q118R, E340K
  • GenBank ID
    XM_015301318.1
  • Entrez Gene
    DCTN1 (a.k.a. Chip150)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Please note that mutations Q118R and E340K were found during Addgene's quality control. The depositor noted that these mutations do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DsRed-p150 217–548 was a gift from Trina Schroer (Addgene plasmid # 51146 ; http://n2t.net/addgene:51146 ; RRID:Addgene_51146)
  • For your References section:

    Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes. Quintyne NJ, Schroer TA. J Cell Biol. 2002 Oct 28;159(2):245-54. Epub 2002 Oct 21. 10.1083/jcb.200203089 PubMed 12391026