Dynamitin-GFP
(Plasmid #51419)

• Purpose: expression of GFP-tagged wild-type dynamitin p50 in mammalian cells
• Depositing Lab: Trina Schroer
• Publication: Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 7500
• Modifications to backbone: Add myc and GFP tag between HindIII and BamHI site of parental pCDNA3 vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dynamitin
• Alt name: DCTN2
• Alt name: Dynactin p50
• Species: G. gallus (chicken)
• Insert Size (bp): 1200
• GenBank ID: NM_001347945.1
• Entrez Gene: DCTN2 (a.k.a. P50)
• Promoter: CMV
• Tags / Fusion Proteins:
  • myc (N terminal on backbone)
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry