pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
(Plasmid
#51247)
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PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEF5-FRT-DEST
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression ; Flpin system
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGli3
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SpeciesM. musculus (mouse)
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Mutationamino acids 849,865,877,907 mutated to Ala
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GenBank ID14634
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Entrez GeneGli3 (a.k.a. Bph, GLI3-190, GLI3FL, Pdn, Xt, add)
- Promoter EF1a
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Tags
/ Fusion Proteins
- 3xHA-TEV (N terminal on insert)
- Flag (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A was a gift from Rajat Rohatgi (Addgene plasmid # 51247 ; http://n2t.net/addgene:51247 ; RRID:Addgene_51247) -
For your References section:
Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling. Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R. Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 10.1016/j.celrep.2013.12.003. 10.1016/j.celrep.2013.12.003 PubMed 24373970