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T7-His-Arp11(1–417)
(Plasmid #51400)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51400 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 4444
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arp11
  • Alt name
    Actr10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1251
  • Entrez Gene
    Actr10 (a.k.a. Actr11, Arp11)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-His-Arp11(1–417) was a gift from Trina Schroer (Addgene plasmid # 51400 ; http://n2t.net/addgene:51400 ; RRID:Addgene_51400)
  • For your References section:

    Interactions between the evolutionarily conserved, actin-related protein, Arp11, actin, and Arp1. Eckley DM, Schroer TA. Mol Biol Cell. 2003 Jul;14(7):2645-54. Epub 2003 Mar 20. 10.1091/mbc.e03-01-0049 PubMed 12857853