pDON-AI-baIaa
(Plasmid #51474)

• Purpose: Express mouse TH2B and TH2A in mammalian cells
• Depositing Lab: Shunsuke Ishii
• Publication: Ishii Lab histone variant plasmids (unpublished)

Backbone

• Vector backbone: pDON-AI
• Backbone manufacturer: TAKARA
• Backbone size w/o insert (bp): 5682
• Total vector size (bp): 7105
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: histone cluster 1, H2aa
• Alt name: Th2a
• Alt name: Hist1h2aa
• Species: M. musculus (mouse)
• Insert Size (bp): 390
• GenBank ID: NM_175658
• Entrez Gene: H2ac1 (a.k.a. Hist1, Hist1h2aa, Th2a)

Gene/Insert 2

• Gene/Insert name: histone cluster 1, H2ba
• Alt name: Th2b
• Alt name: Hist1h2ba
• Species: M. musculus (mouse)
• Insert Size (bp): 382
• GenBank ID: NM_175663
• Entrez Gene: H2bc1 (a.k.a. Hist1h2ba, Th2b)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry