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pRSCgfp-hAIM2
(Plasmid #51666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51666 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 51666-D50 50 μg of DNA in Tris buffer $495
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DNA 51666-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRSC
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 8485
  • Modifications to backbone
    The vector backbone was created by T.C. Tsang et al (Tsang TC, Harris DT, Akporiaye ET, Chu RS, Brailey J, Liu F, Vasanwala FH, Schluter SF, Hersh EM. Mammalian Expression Vector with Two Multiple Cloning Sites for Expression of Two Foreign Genes. Bio Techniques 22, 68, 1997.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    AIM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1032
  • Entrez Gene
    AIM2 (a.k.a. PYHIN4)
  • Promoter CMV

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Alt name
    EGFP
  • Species
    unknown
  • Insert Size (bp)
    720
  • GenBank ID
    unknown
  • Promoter RSV

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSCgfp-hAIM2 was a gift from Emad Alnemri (Addgene plasmid # 51666 ; http://n2t.net/addgene:51666 ; RRID:Addgene_51666)

  • For your References section:

    AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676
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