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pRSCgfp-hAIM2
(Plasmid #51666)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51666 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSC
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 8485
  • Modifications to backbone
    The vector backbone was created by T.C. Tsang et al (Tsang TC, Harris DT, Akporiaye ET, Chu RS, Brailey J, Liu F, Vasanwala FH, Schluter SF, Hersh EM. Mammalian Expression Vector with Two Multiple Cloning Sites for Expression of Two Foreign Genes. Bio Techniques 22, 68, 1997.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    AIM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1032
  • Entrez Gene
    AIM2 (a.k.a. PYHIN4)
  • Promoter CMV

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Alt name
    EGFP
  • Species
    unknown
  • Insert Size (bp)
    720
  • GenBank ID
    unknown
  • Promoter RSV

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSCgfp-hAIM2 was a gift from Emad Alnemri (Addgene plasmid # 51666 ; http://n2t.net/addgene:51666 ; RRID:Addgene_51666)
  • For your References section:

    AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676