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Alpha MHC-DN.JNK2
(Plasmid #51931)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51931 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 51931-D50 50 μg of DNA in Tris buffer $495
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DNA 51931-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    alphaMHC vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DN JNK2
  • Alt name
    JNK2
  • Alt name
    mitogen-activated protein kinase 9
  • Alt name
    MAPK9
  • Species
    M. musculus (mouse)
  • Mutation
    dominant negative: the dual phosphorylation motif TPY was changed to APF
  • Entrez Gene
    Mapk9 (a.k.a. JNK2, Prkm9, p54aSAPK)
  • Promoter cardiac-specific α-myosin heavy chain 5.5 kb promoter
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing found the following additional mutations: S2G, S6C and G8S, they not thought to effect the function of the insert.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Alpha MHC-DN.JNK2 was a gift from Jeffery Molkentin (Addgene plasmid # 51931 ; http://n2t.net/addgene:51931 ; RRID:Addgene_51931)

  • For your References section:

    c-Jun N-terminal kinases (JNK) antagonize cardiac growth through cross-talk with calcineurin-NFAT signaling. Liang Q, Bueno OF, Wilkins BJ, Kuan CY, Xia Y, Molkentin JD. EMBO J. 2003 Oct 1;22(19):5079-89. 10.1093/emboj/cdg474 PubMed 14517246
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