pENO1-dTom-NATr
(Plasmid #52208)

• Purpose: Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker
• Depositing Lab: Robert Wheeler
• Publication: Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. doi: 10.1242/dmm.012039. Epub 2013 May 29.

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 5800
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dTomato
• Species: synthetic construct
• Insert Size (bp): 3135
• Mutation: codon optimized for Candida species
• GenBank ID: AY678268
• Promoter: ENO1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: M13F20
• 3′ sequencing primer: M13Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid insert was custom synthesized.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry