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p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)
(Plasmid #52216)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 52216 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HIF1alpha (401delta603) LCLL
  • Alt name
    HIF1alpha (deltaODD)
  • Alt name
    hypoxia inducible factor 1, alpha subunit
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    aa 401-603 deleted, removes the oxygen-dependent degradation (ODD) domain, and L795V, C800S, L818S, L822V mutations which that disrupt p300/CBP binding
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V) was a gift from Eric Huang (Addgene plasmid # 52216 ; ; RRID:Addgene_52216)
  • For your References section:

    HIF-1alpha induces cell cycle arrest by functionally counteracting Myc. Koshiji M, Kageyama Y, Pete EA, Horikawa I, Barrett JC, Huang LE. EMBO J. 2004 May 5;23(9):1949-56. Epub 2004 Apr 8. 10.1038/sj.emboj.7600196 PubMed 15071503