TRIPZ Human MBD3 shRNA #3 (clone ID V3THS 392210)
(Plasmid
#52354)
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PurposeDoxycycline driven/Tet on expression vector driving shRNA for human MBD3. Inlcudes constitutive Puromycin resistance. RFP reporter is also driven by DOX as a reporter for hairpin induction.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneTRIPZ
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Backbone manufacturerThermo Scientific
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman MBD3 shRNA
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gRNA/shRNA sequenceMBD3
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SpeciesH. sapiens (human)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPurchased from Thermo Scientific
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequencing primer: GGAAAGAATCAAGGAGG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRIPZ Human MBD3 shRNA #3 (clone ID V3THS 392210) was a gift from Jacob Hanna (Addgene plasmid # 52354) -
For your References section:
Deterministic direct reprogramming of somatic cells to pluripotency. Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA, Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z, Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern N, Hanna JH. Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 2013 Sep 18. 10.1038/nature12587 PubMed 24048479