Human Mevalonate diphosphate decarboxylase (MDD)
(Plasmid #52431)

• Purpose: bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)
• Depositing Lab: Henry Miziorko
• Publication: Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):58-67. doi: 10.1016/j.abb.2008.08.024. Epub 2008 Sep 18.

Backbone

• Vector backbone: pET23d(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3666
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mevalonate diphosphate decarboxylase
• Alt name: MDD
• Species: H. sapiens (human)
• Entrez Gene: MVD (a.k.a. FP17780, MPD)
• Promoter: T7
• Tag / Fusion Protein:
  • HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 5’ primer (5’-TTCCCCATGGCCTCGGAGAAGC-3’) encoded an NcoI site harboring the ATG start codon; the 3’ primer (5’-GAGGCTCGAGGGCAGCTGGCTTCGGCAGGC-3’) encoded a XhoI site.