Fast-GCaMP-EF05
(Plasmid #52558)

• Purpose: genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
• Depositing Lab: Samuel Wang
• Publication: Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.

Backbone

• Vector backbone: pJFRC-MUH
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 7831
• Total vector size (bp): 9184
• Modifications to backbone: insert between BglII and NotI
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fast-GCaMP-EF05
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1353
• Mutation: changed from GCaMP3: D397N/G398A/N399D

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: TACT AGATCT GCCGCCACC atgggttctcatcatcat
• 3′ sequencing primer: att cca cca ctg ctc cca

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene 26213

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry